Generator
xynA-PDZli
Part:BBa_K1602035
Designed by: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel Group: iGEM15_TU_Darmstadt (2015-09-16)
Inducible generator of RBS-xynA-PDZlig
This part is capable to generate the protein Xylanase (BBa_K1175005). To fuse the protein to an in vitro scaffold (BBa_K1602027) a linker-ligand sequence was added. This fusion could optimize the enzymatic degradation of Xylose by bringing the respective enzymes in spatial proximity. The ligand of this part binds to the PDZ domain of the scaffold. The construct also contains a strong Ribosome Binding Site (BBa_B0034). |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 134
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 538
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Categories
Parameters
None |