Generator
xynA-PDZli

Part:BBa_K1602035

Designed by: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel   Group: iGEM15_TU_Darmstadt   (2015-09-16)

Inducible generator of RBS-xynA-PDZlig

This part is capable to generate the protein Xylanase (BBa_K1175005). To fuse the protein to an in vitro scaffold (BBa_K1602027) a linker-ligand sequence was added. This fusion could optimize the enzymatic degradation of Xylose by bringing the respective enzymes in spatial proximity. The ligand of this part binds to the PDZ domain of the scaffold. The construct also contains a strong Ribosome Binding Site (BBa_B0034).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 134
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 538


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Categories
Parameters
None